DNA Methylation Biomarkers for Cancer – a Measurement Challenge Although studied for decades, the study of methylated cytosine DNA residues is not a trivial task for clinical in vitro diagnostics The challenge of methylation analysis in clinical diagnostics In the world of diagnostics, the measurement of biological analytes for clinical use trace its [...]
Blood collection tubes prior to centrifuging The difference between plasma and serum for cell-free DNA liquid biopsies Important pre-analytical sampling considerations for DNA and protein biomarker work Using plasma or serum for analysis of novel biomarkers for the early detection of cancer is an effort that spans decades. Thanks to its minimally-invasive nature, [...]
The power of methylation haplotypes How NGS technology informed biology and now transforming cancer diagnostics with an epigenomics Cambrian Explosion Finding a cell-free cancer signal is like finding a needle in a haystack... Of the 28 million CpG residues in the human genome, 60% to 80% of them are methylated at the 5’ [...]
Optimum plasma sample collection and handling for cell-free DNA liquid biopsies A recent publication indicates the best way to preserve intact cell-free DNA for any minimally-invasive genomic assay Liquid biopsy, whether examining cell-free DNA or circulating tumor cells (CTCs), is a very promising approach for non-invasive cancer management. In particular, cell-free DNA approaches in plasma [...]
Screen capture from Dr. Gore's video presentation of the Singlera early-detection technology. Singlera ASHG Presentation now available A video presentation by Dr. Athurva Gore, Assoc. Director of Bioinformatics Singlera Genomics, is now available. The title of his talk is "Circulating tumor DNA methylation haplotypes in plasma can detect cancer four years prior to [...]
Singlera detects single molecules of ctmDNA Single molecule detection of ctmDNA The reason why early detection biomarkers for cancer is not a trivial task First, second and third-generation sequencing First-generation sequencing (also known as Sanger sequencing, or dideoxy-terminator sequencing) was the technology used for the Human Genome Project, and remains a workhorse and [...]
